Which of the following are two major universities located in Kentucky?

The choice of the University of Kentucky and the University of Louisville as two major universities located in Kentucky is based on their prominence within the state and their key roles in higher education. Both institutions are well-established, offering a wide range of undergraduate and graduate programs, and they contribute significantly to the educational, cultural, and economic landscape of Kentucky.

The University of Kentucky, located in Lexington, is the state's flagship university and is known for its research programs, particularly in fields such as healthcare, agriculture, and engineering. The University of Louisville, situated in Louisville, is recognized for its strong programs in nursing, business, and law, among others. Both universities serve diverse student populations and have significant contributions to local and statewide initiatives, making them central to Kentucky's higher education system.

In contrast, the other choices include universities from outside Kentucky, such as those from Tennessee, Michigan, Ohio, and California, which do not pertain to the query regarding major universities within the state of Kentucky.